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cmv rras plasmid  (Addgene inc)


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    Addgene inc cmv rras plasmid
    a Violin plots show decreased R-RAS mRNA in EC of IPF ( n = 4 lungs) compared to donor ( n = 8 lungs), GSE 122960 datasets. R-RAS expression was log normalized. b R-RAS mRNA was decreased in FACS-sorted ECs from IPF lungs ( n = 6) compared to donor lungs ( n = 3) as shown by qRT-PCR. Data presented as mean ± SD, * p = 0.238, Mann–Whitney Two-tailed test. c Immunostaining for R-RAS, FOXF1 and CD31 shows co-localization of FOXF1 and R-RAS in ECs of donor lungs ( n = 5). Neither R-RAS nor FOXF1 are detected in ECs within IPF fibrotic lesions ( n = 5). Nuclei are counterstained with DAPI. Bar = 20 μm. d qRT-PCR shows that shRNA-mediated knockdown of FOXF1 (sh FOXF1 ) in HUVECs decreased R-RAS mRNA compared to control (Scr). ACTB mRNA was used for normalization ( n = 3), ** p < 0.01 and *** p < 0.001 by Student’s T test (two-tailed). e qRT-PCR shows that siRNA-mediated knockdown of Foxf1 in mouse MFLM-91U ECs decreased <t>Rras</t> mRNA. Actb mRNA was used for normalization ( n = 3). f ChIP-seq shows direct binding of FOXF1 protein to Rras promoter region in MFLM-91U cells. Binding of FOXF1 to Rras promoter is associated with H3K4me3 marks but not H3K27me3 marks. g Schematic drawing of the pGL2-Rras-Luc construct with the −762/+13 bp Rras promoter region containing the FOXF1-binding site (top panel). In co-transfection experiments, <t>CMV-FOXF1</t> expression vector increased transcriptional activity of the −762/+13 Rras promoter region compared to CMV-empty vector (bottom panel), n = 3, *** p < 0.001. h Overexpression of R-Ras decreased Ccl2 and TNFα mRNAs in mock-transfected cells and prevented upregulation of Ccl2 and TNFα in cells transfected with Foxf1-specific siRNA. MFLM-91U cells were transfected with non-targeting siRNA (siControl) or si Foxf1 , and EEV-empty vector or EEV- Rras . Actb mRNA was used for normalization (siControl+EEV-empty Foxf1 , n = 6; siControl+EEV-empty Rras , n = 6; siControl+EEV-empty Ccl2 , n = 3; siControl+EEV-empty Tnf , n = 6; siControl+EEV-Rras Foxf1 , n = 6; siControl+EEV-Rras Rras , n = 6; siControl+EEV-Rras Ccl2 , n = 6; siControl+EEV-Rras Tnf , n = 3; siFoxf1+EEV-empty Foxf1 , n = 6; siFoxf1+EEV-empty Rras , n = 3; siFoxf1+EEV-empty Ccl2 , n = 3; siFoxf1+EEV-empty Tnf , n = 3; siFoxf1+EEV-Rras Foxf1 , n = 6; siFoxf1l+EEV-Rras Rras , n = 3; siFoxf1l+EEV-Rras Ccl2 , n = 6; siFoxf1+EEV-Rras Tnf , n = 3), * p < 0.05, ** p < 0.01, *** p < 0.001 by Student’s t test (two tailed). Source data are provided as a Source Data file.
    Cmv Rras Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cmv+rras+plasmid/pLEX307-mFzd1+(Plasmid+%23102864)/pmc10156846-391-13-16
    Average 91 stars, based on 1 article reviews
    cmv rras plasmid - by Bioz Stars, 2026-10
    91/100 stars

    Images

    1) Product Images from "Lung endothelial cells regulate pulmonary fibrosis through FOXF1/R-Ras signaling"

    Article Title: Lung endothelial cells regulate pulmonary fibrosis through FOXF1/R-Ras signaling

    Journal: Nature Communications

    doi: 10.1038/s41467-023-38177-2

    a Violin plots show decreased R-RAS mRNA in EC of IPF ( n = 4 lungs) compared to donor ( n = 8 lungs), GSE 122960 datasets. R-RAS expression was log normalized. b R-RAS mRNA was decreased in FACS-sorted ECs from IPF lungs ( n = 6) compared to donor lungs ( n = 3) as shown by qRT-PCR. Data presented as mean ± SD, * p = 0.238, Mann–Whitney Two-tailed test. c Immunostaining for R-RAS, FOXF1 and CD31 shows co-localization of FOXF1 and R-RAS in ECs of donor lungs ( n = 5). Neither R-RAS nor FOXF1 are detected in ECs within IPF fibrotic lesions ( n = 5). Nuclei are counterstained with DAPI. Bar = 20 μm. d qRT-PCR shows that shRNA-mediated knockdown of FOXF1 (sh FOXF1 ) in HUVECs decreased R-RAS mRNA compared to control (Scr). ACTB mRNA was used for normalization ( n = 3), ** p < 0.01 and *** p < 0.001 by Student’s T test (two-tailed). e qRT-PCR shows that siRNA-mediated knockdown of Foxf1 in mouse MFLM-91U ECs decreased Rras mRNA. Actb mRNA was used for normalization ( n = 3). f ChIP-seq shows direct binding of FOXF1 protein to Rras promoter region in MFLM-91U cells. Binding of FOXF1 to Rras promoter is associated with H3K4me3 marks but not H3K27me3 marks. g Schematic drawing of the pGL2-Rras-Luc construct with the −762/+13 bp Rras promoter region containing the FOXF1-binding site (top panel). In co-transfection experiments, CMV-FOXF1 expression vector increased transcriptional activity of the −762/+13 Rras promoter region compared to CMV-empty vector (bottom panel), n = 3, *** p < 0.001. h Overexpression of R-Ras decreased Ccl2 and TNFα mRNAs in mock-transfected cells and prevented upregulation of Ccl2 and TNFα in cells transfected with Foxf1-specific siRNA. MFLM-91U cells were transfected with non-targeting siRNA (siControl) or si Foxf1 , and EEV-empty vector or EEV- Rras . Actb mRNA was used for normalization (siControl+EEV-empty Foxf1 , n = 6; siControl+EEV-empty Rras , n = 6; siControl+EEV-empty Ccl2 , n = 3; siControl+EEV-empty Tnf , n = 6; siControl+EEV-Rras Foxf1 , n = 6; siControl+EEV-Rras Rras , n = 6; siControl+EEV-Rras Ccl2 , n = 6; siControl+EEV-Rras Tnf , n = 3; siFoxf1+EEV-empty Foxf1 , n = 6; siFoxf1+EEV-empty Rras , n = 3; siFoxf1+EEV-empty Ccl2 , n = 3; siFoxf1+EEV-empty Tnf , n = 3; siFoxf1+EEV-Rras Foxf1 , n = 6; siFoxf1l+EEV-Rras Rras , n = 3; siFoxf1l+EEV-Rras Ccl2 , n = 6; siFoxf1+EEV-Rras Tnf , n = 3), * p < 0.05, ** p < 0.01, *** p < 0.001 by Student’s t test (two tailed). Source data are provided as a Source Data file.
    Figure Legend Snippet: a Violin plots show decreased R-RAS mRNA in EC of IPF ( n = 4 lungs) compared to donor ( n = 8 lungs), GSE 122960 datasets. R-RAS expression was log normalized. b R-RAS mRNA was decreased in FACS-sorted ECs from IPF lungs ( n = 6) compared to donor lungs ( n = 3) as shown by qRT-PCR. Data presented as mean ± SD, * p = 0.238, Mann–Whitney Two-tailed test. c Immunostaining for R-RAS, FOXF1 and CD31 shows co-localization of FOXF1 and R-RAS in ECs of donor lungs ( n = 5). Neither R-RAS nor FOXF1 are detected in ECs within IPF fibrotic lesions ( n = 5). Nuclei are counterstained with DAPI. Bar = 20 μm. d qRT-PCR shows that shRNA-mediated knockdown of FOXF1 (sh FOXF1 ) in HUVECs decreased R-RAS mRNA compared to control (Scr). ACTB mRNA was used for normalization ( n = 3), ** p < 0.01 and *** p < 0.001 by Student’s T test (two-tailed). e qRT-PCR shows that siRNA-mediated knockdown of Foxf1 in mouse MFLM-91U ECs decreased Rras mRNA. Actb mRNA was used for normalization ( n = 3). f ChIP-seq shows direct binding of FOXF1 protein to Rras promoter region in MFLM-91U cells. Binding of FOXF1 to Rras promoter is associated with H3K4me3 marks but not H3K27me3 marks. g Schematic drawing of the pGL2-Rras-Luc construct with the −762/+13 bp Rras promoter region containing the FOXF1-binding site (top panel). In co-transfection experiments, CMV-FOXF1 expression vector increased transcriptional activity of the −762/+13 Rras promoter region compared to CMV-empty vector (bottom panel), n = 3, *** p < 0.001. h Overexpression of R-Ras decreased Ccl2 and TNFα mRNAs in mock-transfected cells and prevented upregulation of Ccl2 and TNFα in cells transfected with Foxf1-specific siRNA. MFLM-91U cells were transfected with non-targeting siRNA (siControl) or si Foxf1 , and EEV-empty vector or EEV- Rras . Actb mRNA was used for normalization (siControl+EEV-empty Foxf1 , n = 6; siControl+EEV-empty Rras , n = 6; siControl+EEV-empty Ccl2 , n = 3; siControl+EEV-empty Tnf , n = 6; siControl+EEV-Rras Foxf1 , n = 6; siControl+EEV-Rras Rras , n = 6; siControl+EEV-Rras Ccl2 , n = 6; siControl+EEV-Rras Tnf , n = 3; siFoxf1+EEV-empty Foxf1 , n = 6; siFoxf1+EEV-empty Rras , n = 3; siFoxf1+EEV-empty Ccl2 , n = 3; siFoxf1+EEV-empty Tnf , n = 3; siFoxf1+EEV-Rras Foxf1 , n = 6; siFoxf1l+EEV-Rras Rras , n = 3; siFoxf1l+EEV-Rras Ccl2 , n = 6; siFoxf1+EEV-Rras Tnf , n = 3), * p < 0.05, ** p < 0.01, *** p < 0.001 by Student’s t test (two tailed). Source data are provided as a Source Data file.

    Techniques Used: Expressing, Quantitative RT-PCR, MANN-WHITNEY, Two Tailed Test, Immunostaining, shRNA, Knockdown, Control, ChIP-sequencing, Binding Assay, Construct, Cotransfection, Plasmid Preparation, Activity Assay, Over Expression, Transfection

    Related Articles

    In Vitro:

    Article Title: Lung endothelial cells regulate pulmonary fibrosis through FOXF1/R-Ras signaling
    Article Snippet: .. In vitro rescue experiments were performed by dual transfection of si Foxf1 and CMV- Rras plasmid (Addgene plasmid # 102864) into MFLM-91U cells using Dharmafect Duo transfection reagent (Dharmacon). ..

    Transfection:

    Article Title: Lung endothelial cells regulate pulmonary fibrosis through FOXF1/R-Ras signaling
    Article Snippet: .. In vitro rescue experiments were performed by dual transfection of si Foxf1 and CMV- Rras plasmid (Addgene plasmid # 102864) into MFLM-91U cells using Dharmafect Duo transfection reagent (Dharmacon). ..

    Plasmid Preparation:

    Article Title: Lung endothelial cells regulate pulmonary fibrosis through FOXF1/R-Ras signaling
    Article Snippet: .. In vitro rescue experiments were performed by dual transfection of si Foxf1 and CMV- Rras plasmid (Addgene plasmid # 102864) into MFLM-91U cells using Dharmafect Duo transfection reagent (Dharmacon). ..



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    cmv rras plasmid  (Addgene inc)
    91
    Addgene inc cmv rras plasmid
    a Violin plots show decreased R-RAS mRNA in EC of IPF ( n = 4 lungs) compared to donor ( n = 8 lungs), GSE 122960 datasets. R-RAS expression was log normalized. b R-RAS mRNA was decreased in FACS-sorted ECs from IPF lungs ( n = 6) compared to donor lungs ( n = 3) as shown by qRT-PCR. Data presented as mean ± SD, * p = 0.238, Mann–Whitney Two-tailed test. c Immunostaining for R-RAS, FOXF1 and CD31 shows co-localization of FOXF1 and R-RAS in ECs of donor lungs ( n = 5). Neither R-RAS nor FOXF1 are detected in ECs within IPF fibrotic lesions ( n = 5). Nuclei are counterstained with DAPI. Bar = 20 μm. d qRT-PCR shows that shRNA-mediated knockdown of FOXF1 (sh FOXF1 ) in HUVECs decreased R-RAS mRNA compared to control (Scr). ACTB mRNA was used for normalization ( n = 3), ** p < 0.01 and *** p < 0.001 by Student’s T test (two-tailed). e qRT-PCR shows that siRNA-mediated knockdown of Foxf1 in mouse MFLM-91U ECs decreased <t>Rras</t> mRNA. Actb mRNA was used for normalization ( n = 3). f ChIP-seq shows direct binding of FOXF1 protein to Rras promoter region in MFLM-91U cells. Binding of FOXF1 to Rras promoter is associated with H3K4me3 marks but not H3K27me3 marks. g Schematic drawing of the pGL2-Rras-Luc construct with the −762/+13 bp Rras promoter region containing the FOXF1-binding site (top panel). In co-transfection experiments, <t>CMV-FOXF1</t> expression vector increased transcriptional activity of the −762/+13 Rras promoter region compared to CMV-empty vector (bottom panel), n = 3, *** p < 0.001. h Overexpression of R-Ras decreased Ccl2 and TNFα mRNAs in mock-transfected cells and prevented upregulation of Ccl2 and TNFα in cells transfected with Foxf1-specific siRNA. MFLM-91U cells were transfected with non-targeting siRNA (siControl) or si Foxf1 , and EEV-empty vector or EEV- Rras . Actb mRNA was used for normalization (siControl+EEV-empty Foxf1 , n = 6; siControl+EEV-empty Rras , n = 6; siControl+EEV-empty Ccl2 , n = 3; siControl+EEV-empty Tnf , n = 6; siControl+EEV-Rras Foxf1 , n = 6; siControl+EEV-Rras Rras , n = 6; siControl+EEV-Rras Ccl2 , n = 6; siControl+EEV-Rras Tnf , n = 3; siFoxf1+EEV-empty Foxf1 , n = 6; siFoxf1+EEV-empty Rras , n = 3; siFoxf1+EEV-empty Ccl2 , n = 3; siFoxf1+EEV-empty Tnf , n = 3; siFoxf1+EEV-Rras Foxf1 , n = 6; siFoxf1l+EEV-Rras Rras , n = 3; siFoxf1l+EEV-Rras Ccl2 , n = 6; siFoxf1+EEV-Rras Tnf , n = 3), * p < 0.05, ** p < 0.01, *** p < 0.001 by Student’s t test (two tailed). Source data are provided as a Source Data file.
    Cmv Rras Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cmv+rras+plasmid/pLEX307-mFzd1+(Plasmid+%23102864)/pmc10156846-391-13-16
    Average 91 stars, based on 1 article reviews
    cmv rras plasmid - by Bioz Stars, 2026-10
    91/100 stars
    		  Buy from Supplier

    Image Search Results


    a Violin plots show decreased R-RAS mRNA in EC of IPF ( n = 4 lungs) compared to donor ( n = 8 lungs), GSE 122960 datasets. R-RAS expression was log normalized. b R-RAS mRNA was decreased in FACS-sorted ECs from IPF lungs ( n = 6) compared to donor lungs ( n = 3) as shown by qRT-PCR. Data presented as mean ± SD, * p = 0.238, Mann–Whitney Two-tailed test. c Immunostaining for R-RAS, FOXF1 and CD31 shows co-localization of FOXF1 and R-RAS in ECs of donor lungs ( n = 5). Neither R-RAS nor FOXF1 are detected in ECs within IPF fibrotic lesions ( n = 5). Nuclei are counterstained with DAPI. Bar = 20 μm. d qRT-PCR shows that shRNA-mediated knockdown of FOXF1 (sh FOXF1 ) in HUVECs decreased R-RAS mRNA compared to control (Scr). ACTB mRNA was used for normalization ( n = 3), ** p < 0.01 and *** p < 0.001 by Student’s T test (two-tailed). e qRT-PCR shows that siRNA-mediated knockdown of Foxf1 in mouse MFLM-91U ECs decreased Rras mRNA. Actb mRNA was used for normalization ( n = 3). f ChIP-seq shows direct binding of FOXF1 protein to Rras promoter region in MFLM-91U cells. Binding of FOXF1 to Rras promoter is associated with H3K4me3 marks but not H3K27me3 marks. g Schematic drawing of the pGL2-Rras-Luc construct with the −762/+13 bp Rras promoter region containing the FOXF1-binding site (top panel). In co-transfection experiments, CMV-FOXF1 expression vector increased transcriptional activity of the −762/+13 Rras promoter region compared to CMV-empty vector (bottom panel), n = 3, *** p < 0.001. h Overexpression of R-Ras decreased Ccl2 and TNFα mRNAs in mock-transfected cells and prevented upregulation of Ccl2 and TNFα in cells transfected with Foxf1-specific siRNA. MFLM-91U cells were transfected with non-targeting siRNA (siControl) or si Foxf1 , and EEV-empty vector or EEV- Rras . Actb mRNA was used for normalization (siControl+EEV-empty Foxf1 , n = 6; siControl+EEV-empty Rras , n = 6; siControl+EEV-empty Ccl2 , n = 3; siControl+EEV-empty Tnf , n = 6; siControl+EEV-Rras Foxf1 , n = 6; siControl+EEV-Rras Rras , n = 6; siControl+EEV-Rras Ccl2 , n = 6; siControl+EEV-Rras Tnf , n = 3; siFoxf1+EEV-empty Foxf1 , n = 6; siFoxf1+EEV-empty Rras , n = 3; siFoxf1+EEV-empty Ccl2 , n = 3; siFoxf1+EEV-empty Tnf , n = 3; siFoxf1+EEV-Rras Foxf1 , n = 6; siFoxf1l+EEV-Rras Rras , n = 3; siFoxf1l+EEV-Rras Ccl2 , n = 6; siFoxf1+EEV-Rras Tnf , n = 3), * p < 0.05, ** p < 0.01, *** p < 0.001 by Student’s t test (two tailed). Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Lung endothelial cells regulate pulmonary fibrosis through FOXF1/R-Ras signaling

    doi: 10.1038/s41467-023-38177-2

    Figure Lengend Snippet: a Violin plots show decreased R-RAS mRNA in EC of IPF ( n = 4 lungs) compared to donor ( n = 8 lungs), GSE 122960 datasets. R-RAS expression was log normalized. b R-RAS mRNA was decreased in FACS-sorted ECs from IPF lungs ( n = 6) compared to donor lungs ( n = 3) as shown by qRT-PCR. Data presented as mean ± SD, * p = 0.238, Mann–Whitney Two-tailed test. c Immunostaining for R-RAS, FOXF1 and CD31 shows co-localization of FOXF1 and R-RAS in ECs of donor lungs ( n = 5). Neither R-RAS nor FOXF1 are detected in ECs within IPF fibrotic lesions ( n = 5). Nuclei are counterstained with DAPI. Bar = 20 μm. d qRT-PCR shows that shRNA-mediated knockdown of FOXF1 (sh FOXF1 ) in HUVECs decreased R-RAS mRNA compared to control (Scr). ACTB mRNA was used for normalization ( n = 3), ** p < 0.01 and *** p < 0.001 by Student’s T test (two-tailed). e qRT-PCR shows that siRNA-mediated knockdown of Foxf1 in mouse MFLM-91U ECs decreased Rras mRNA. Actb mRNA was used for normalization ( n = 3). f ChIP-seq shows direct binding of FOXF1 protein to Rras promoter region in MFLM-91U cells. Binding of FOXF1 to Rras promoter is associated with H3K4me3 marks but not H3K27me3 marks. g Schematic drawing of the pGL2-Rras-Luc construct with the −762/+13 bp Rras promoter region containing the FOXF1-binding site (top panel). In co-transfection experiments, CMV-FOXF1 expression vector increased transcriptional activity of the −762/+13 Rras promoter region compared to CMV-empty vector (bottom panel), n = 3, *** p < 0.001. h Overexpression of R-Ras decreased Ccl2 and TNFα mRNAs in mock-transfected cells and prevented upregulation of Ccl2 and TNFα in cells transfected with Foxf1-specific siRNA. MFLM-91U cells were transfected with non-targeting siRNA (siControl) or si Foxf1 , and EEV-empty vector or EEV- Rras . Actb mRNA was used for normalization (siControl+EEV-empty Foxf1 , n = 6; siControl+EEV-empty Rras , n = 6; siControl+EEV-empty Ccl2 , n = 3; siControl+EEV-empty Tnf , n = 6; siControl+EEV-Rras Foxf1 , n = 6; siControl+EEV-Rras Rras , n = 6; siControl+EEV-Rras Ccl2 , n = 6; siControl+EEV-Rras Tnf , n = 3; siFoxf1+EEV-empty Foxf1 , n = 6; siFoxf1+EEV-empty Rras , n = 3; siFoxf1+EEV-empty Ccl2 , n = 3; siFoxf1+EEV-empty Tnf , n = 3; siFoxf1+EEV-Rras Foxf1 , n = 6; siFoxf1l+EEV-Rras Rras , n = 3; siFoxf1l+EEV-Rras Ccl2 , n = 6; siFoxf1+EEV-Rras Tnf , n = 3), * p < 0.05, ** p < 0.01, *** p < 0.001 by Student’s t test (two tailed). Source data are provided as a Source Data file.

    Article Snippet: In vitro rescue experiments were performed by dual transfection of si Foxf1 and CMV- Rras plasmid (Addgene plasmid # 102864) into MFLM-91U cells using Dharmafect Duo transfection reagent (Dharmacon).

    Techniques: Expressing, Quantitative RT-PCR, MANN-WHITNEY, Two Tailed Test, Immunostaining, shRNA, Knockdown, Control, ChIP-sequencing, Binding Assay, Construct, Cotransfection, Plasmid Preparation, Activity Assay, Over Expression, Transfection